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Title: Plasmids-Mediated Antibiotic Resistance in E. coli
Author(s): Nariman A. H. Aly; Amany Tharwat and Wesam F. El-Baz
Source: Arab Journal of Biotechnology, 1998, Vol. 1, No. 1,, 17-27

Abstract: In this study, E. coli strains were isolated from thirty hospitalized patients receiving antimicrobials, thirty not on antimicrobials and thirty healthy persons were used as controls. Antimicrobial susceptibility test was done to thirteen of the commonly used antimicrobials in hospitals. There was a significant increase in the percentage of E. coli resistant to most of the antimicrobials on comparing the patients receiving antimicrobials and control group. Among the 14 E. coli strains tested, plasmid profile analysis of resistant strains was done and plasmid numbers ranged from 0 to 8 indigenous plasmids with variable sizes. Curing and transformation experiments revealed the existence of CTXr, CROr, CFBr and OFXr genes on plasmids. Among these plasmids, the OFXr plasmids of strains 1 and 14 were found to be conjugative...

Addresses: Microbial Genetics Dept., Genetic Engineering and Biotechnology Div., National Research Centre, Cairo, Egypt. Departments of Microbiology & Immunology and Internal Medicine, Faculty of Medicine, Ain Shams Univ., Cairo, Egypt...

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Country Code: EG