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Title: Molecular Cloning, Purification and Characterization of Schistosoma mansoni Fimbrin
Author(s): M. A. Saber and Hanan A. Hamid
Source: Arab Journal of Biotechnology, 1998, Vol. 1, No. 1,, 1-16

Abstract: Screening of a Schistosoma mansoni cDNA expression library with sera from patients with chronic S. mansoni infections (SCI) resulted in isolation of a clone containing a 2.4Kb cDNA insert. An open reading frame (ORF) of 2Kb coded for a 651 deduced amino sequence that showed a striking homology with chicken fimbrin and human plastin. The insert was ligated into the pET-3a expression vector and was used to express a 70 kDa fusion protein S. mansoni fimbrin (Smfim). Smfim was purified from bacterial proteins using cation exchange chromatography. Antibodies against purified Smfim protein was raised in rabbits, and IgG fraction was purified on protein G-Sepharose column. The expressed protein was recognized by SCI, mouse sera from immunized mice (UV-irradiated cercaria), and from vaccinated mice (against worm homogenate). Smfim was localized in the tegumental region of adult S. mansoni worms as detected using immunofluorescence. The role of Smfim protein as a protective antigen was assessed by immunization of mice...

Addresses: Department of Biochemistry, Theodore Bilharz Research Institute,12411, Imbaba, Giza, Egypt...

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Country Code: EG