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Title: Growth of Date Palm Callus as Affected by Growth Regulators, Sugars as Carbon Source and Amino Acids as Organic Nitrogen Source
Author(s): E. A. Abdel-Rahim; O. M. Abdel-Fatah; M. I. Kobasse; H. A. El-Shemy and M. B. Abd El-Samei
Source: Arab Journal of Biotechnology, 1998, Vol. 1, No. 1,, 99-106

Abstract: Fresh weight, dry weight and growth rate of callus derived from leaf primordia explants of date palm were determined as affected by casein hydrolysate, growth regulators, soluble sugars, and amino acids. MS-medium that contained kinetin 0.5 mg/L with 2 mg/L 2,4-D was the best combination of growth regulators in callus production. It was found that BA and kinetin gave the best growth, but 2,4-D failed to stimulate callus growth. The results of soluble sugars showed that, fresh weight of callus was increased with sucrose, fructose and glucose, and decreased with mannitol. The results revealed that all amino acid treatments steadily decreased both fresh and dry weights of calli compared with the control, while they were gradually increased with casein hydrolysate...

Addresses: Agric. Biochem. Dept., Fac. Agric., Cairo Univ., Giza, Egypt. Agric. Chem. Dept., Fac. Agric., Minia Univ., El-Minia, Egypt...

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Country Code: EG

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