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Title: Development of Genotype-Independent and Efficient Regeneration Procedure for Flax (Linum usitatissimum L.)
Author(s): M. Koronfel
Source: Arab Journal of Biotechnology, 1998, Vol. 1, No. 1,, 87-92

Abstract: A genotype-independent and efficient regeneration procedure for flax (Linum usitatissimum L.) was developed. Egyptian and Canadian flax explants (hypocotyls from 5-days-old seedling) rapidly proliferated to provide easy-to-root shoots within a few days on MS basal medium with Gamborg's vitamins and supplemented by 1.0 ppm kinetin and maintained in a controlled growth cabinet for a 16-h photoperiod at 20 ± 0.5oC with a light intensity of about 2000 lux provided by cool white fluorescent tubes. The formation of complete plants was then possible by transferring the shoot tips to hormone-free nutrient agar medium for rooting. This procedure was consistently the best for the four studied Egyptian cultivars and one Canadian cultivar. Besides rapid, genotypeindependent and efficient regeneration frequency, the present procedure has another advantage of avoiding callus formation, thereby reducing the incidence of somaclonal variation. This direct regeneration can be used for the vegetative propagation or cloning of a particular line of flax and for genetic transformation of flax...

Addresses: Agronomy Department, Faculty of Agriculture, Cairo University. Giza, Egypt...

FullText: Click Here to access the full text
Country Code: EG

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